Or Iota, the smallest letter of the Greek alphabet, used metaphorically or proverbially for the smallest thing (Matt. 5:18); or it may be = yod, which is the smallest of the Hebrew letters.
Jot
1 verse
Matthew
Matthew 5:18
For verily I say unto you, Till heaven and earth pass, one jot or one tittle shall in no wise pass from the law, till all be fulfilled.
From Easton's Bible Dictionary
Matthew George Easton, 1897
From Smith's Bible Dictionary
William Smith, 1884
the English form of the Greek iota , i.e., the smallest letter of the Greek alphabet. The Hebrew is yod , or y formed like a comma (). It is used metaphorically to express the minutest thing.